The present study was conducted to find the effect of organic and inorganic Se on the semen quality of roosters. Three experimental groups were constituted with total of 24 males (these groups) supplemented with organic Se (0.35 mg/kg), inorganic Se (0.35 mg/kg) and without any supplementation (control). The results revealed that Se supplementation in both form improved sperm concentration and total number sperm per ejaculate with no effect on the semen volume. Further semen quality was not affected by the age of the roosters. Additionally, organic Se proved to be the superior form in comparison with inorganic Se.