The effect of adding orange
juice into the extender of cocks' semen on mass motility, individual
motility, dead and abnormal spermatozoa and acrosomal abnormalities were
studied. A total of 60 White layer cocks, 32 weeks of age, were randomly
divided into 6 groups of 10 cocks in this experiment. Semen samples for
each treatment group were collected on a weekly basis during the whole
experimental period which lasted 8 weeks. The treatment groups were; T1-
the control (fresh semen); T2- the semen extended 1 : 1 with Al-Daraji 2
extender (AD2E) alone, whereas T3, T4, T5 and T6 represented semen
samples extended with AD2E extender and supplemented with 1, 4, 7 or 10
ml of orange juice/100 ml extender. Results revealed that after 0, 24,
48 or 72 h in vitro storage, the supplementation of cocks semen
extender with 7 and 10 ml orange juice/100 ml of extender (T5, T6)
caused significant (P<0.05) increases in mass motility and individual
motility of spermatozoa and significant decreases (P<0.05) in
percentages of dead spermatozoa, abnormal spermatozoa and acrosomal
abnormalities compared with control group. However, T2, T3 and T4 groups
showed significant improvements in all of theses parameters in
comparison with control group. Furthermore, there were no significant
differences between T5 and T6 and between T2, T3 and T4 as regards all
characteristics included in this study. In conclusion, the
supplementation of orange juice into semen extender plays an important
role in protecting spermatozoa against the harmful effects of lipid
peroxidation during in vitro storage of cocks' semen for up to 72
h.
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