A research was conducted to explore the effects of various levels of L-Carnitine in diet on carcass characteristics and some serum parameters in female broiler chickens. A total of 480 one-day old female broiler chicken (Ross 308) were randomly allocated to 4 dietary treatments (6 replicates with 20 birds in each) in a completely randomized design. Treatments included were control and the addition of various levels of L-Carnitine at level of 100, 200 and 300 mg/kg in diets. The results showed that 300 mg/kg L-Carnitine in diet decreased live body weight, carcass, thigh, and intestinal weight (P<0.05). However, all dietary treatments decreased breast weight compared to control diet (P<0.05). In addition, no significant difference was observed in carcass yield and relative organ weights. The serum triglyceride decreased significantly in treated groups. Aspartate aminotransferase (AST), alanine aminotransferase (ALT) and albumin increased significantly (P<0.05) in group fed 300 mg/kg L-carnitine. In conclusion, the addition of L-Carnitine in diets had no positive effects on carcass traits in first 10 days of age.