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PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
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Expression of functional leptin receptor (OB-Rb) mRNA in the sheep
tissues |
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Abbas Abavisani 1*, Hesam Dehghani 1 and Morteza
Zahedi2 |
1Division
of Physiology, Department of Basic Sciences, Faculty of Veterinary
Medicine and Institute of Biotechnology, Ferdowsi University of Mashhad,
Mashhad, Iran; 2Department of Basic Sciences, Veterinary
Faculty, Ferdowsi University of Mashhad, Mashhad, Iran |
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Abstract |
Leptin, a 167-amino acid hormone, is secreted mainly by fat tissue.
Leptin signaling plays crucial roles on the regulation of metabolism and
reproductive function through endocrine and probably paracrine
mechanisms. So, detection of leptin receptor in various tissues is
essential to an understanding of leptin physiology in sheep. The aim of
the present study was to investigate expression pattern of functional
ovine leptin receptor (OB-Rb) in adult tissues using reverse
transcription and polymerase chain reaction analysis (RT-PCR).
Ovine beta actin gene was chosen as an
internal positive control as well as RNA purification marker.
RT-PCR experiments confirmed the expression of functional leptin
receptor mRNA in ovine tissues including fat, liver, rumen and lung, but
not in kidney and hypophysis.
Characterization of functional leptin receptor in different organs
suggests that leptin has paracrine/ autocrine effects on many target
organs as well as their physiology in sheep which should be more
investigated in the future.
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Keywords:
Sheep; leptin receptor mRNA (OB-Rb); tissues, RT-PCR |
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To cite this article:
Abavisani A, Dehghani H and Zahedi M,
2012.
Expression of functional leptin receptor (OB-Rb) mRNA in the sheep
tissues.
Res.
Opin. Anim. Vet. Sci., 2(7), 449-453. |
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