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Developmental competence of water buffalo oocytes in
a synthetic oviductal fluid base medium
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Lerma C. Ocampo1, 2, Elfren F. Celestino
Jr3, Flocerfida P. Aquino1 and Marlon
B. Ocampo1,3 |
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1Philippine Carabao Center, Reproductive
Biotechnology Unit, Science City of Muñoz, Nueva Ecija, Philippines,
2Department of Biological Sciences, Central Luzon State
University, Science City of Muñoz, Nueva Ecija, 3College of
Veterinary Science and Medicine, Central Luzon State University, Science
City of Muñoz, Nueva Ecija, Philippines, 3120
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| Abstract |
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In this study,
in vitro derived (oocytes from
ovaries of slaughtered buffalo cows) and
in vivo derived (oocytes
collected through TUFA from living buffalo cows) oocytes were assessed
on their ability to acquire the necessary developmental competence in
the production of blastocyst stage embryos when cultured in culture
systems using a complex/serial culture media or a single based medium
from maturation to culture. In
vitro derived oocytes ability to complete the 1st meiotic
division (nuclear maturation) showed no difference when cultured in
either culture systems (82.6-83.1%) but was higher compared to
in vivo derived oocytes
(62.3-74.4%). The cleavage rate of
in vivo derived oocytes (78.7-79.2%) was significantly higher than
in vitro derived oocytes
(59.4-62.9%). Significantly higher blastocyst formation rate was
observed from in vivo derived
oocytes cultured in a single based culture system (31.1%). Overall, the
results showed the usefulness and practicality of using a single
synthetic oviductal fluid based medium for supporting buffalo oocytes
acquisition of developmental competence in an
in vitro culture system.
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Keywords:
Synthetic oviductal fluid;
maturation; embryo development
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| To cite this article:
Ocampo LC, EF Celestino Jr,
FP Aquino and MB Ocampo,
2016.
Developmental competence
of water buffalo oocytes in a synthetic oviductal fluid base medium.
Res.
Opin. Anim. Vet. Sci., 6(6): 173-177. |
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