The response of broiler chickens was investigated to Natuzyme Plus^® with canola meal. A total of 380 unsexed 1-d-old broiler chickens (Ross 308) assigned to a 2 × 3 factorial arrangement as completely randomized design with 6 treatments. Treatments were inclusion of canola meals (0, 6, or 12%) and enzyme (with 0.35 g/kg Natuzyme Plus^® or without enzyme) in broiler chicken diets. Body weight gain of broiler chickens decreased by the inclusion of 6 and 12% of canola meal in diets, while the feed conversion ratio improved by the inclusion of 12% of canola meal in diets from 1 to 21 d of age (P<0.05). Dietary inclusion of enzyme increased body weight gain of broiler chickens (P<0.05). Except control, the inclusion of enzyme in diets containing canola meal increased body weight gain rather diets without the enzyme (P<0.05). The inclusion of enzyme in broiler chicken diets increased ileal digestibility of dry matter, organic matter, and crude protein (P<0.05). Natuzyme-supplemented diets containing canola meal improved growth performance slightly compared with un-supplemented diets. The use of canola meal at a level of 6% and canola meal with the enzyme at a level of 12% was suitable.