The aim of this study was to identify single nucleotide polymorphisms of heat shock protein (hsp70) at exon region in Ross 308 commercial broiler strains during 6 to 7 week of age. Hsp70 coding region was amplified by PCR technique using specific primers with 359bp. Quality was performed by loading in an agarose gel and single nucleotide polymorphism was identified by direct sequencing technique. The results of the sequences were determined by using the Multi-Align and Chromas software's and possible single nucleotide polymorphisms were detected among the different samples. A comparison of the sequence of samples specified in the Multi-Align showed that sequences of samples are different in four specific loci. Sequence comparison of different samples by Multi-Align revealed that mutations happened at G36, G44, G132 and G335 loci. Sequence was compared with obtained data from NCBI database using NCBI Blast-Multi-Align site. The results showed missing T and A at locus G36 and locus G44 respectively. Also the transition of G to A at locus G132 and C to G at locus G335 was observed. The frequency of A and G at G132 is 0.3438 and 0.6265 respectively, also the frequency of G and C at G335 was 0.3312 and 0.6687 respectively. The results show that there is polymorphism in hsp70 in Ross308 which can be used as a tool in future breeding program.