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PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
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Genetic differentiation
and gene flow between four Tunisian sheep populations (Ovis aries)
using random amplified polymorphic DNA-polymerase chain reaction
(RAPD-PCR) analysis |
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Haifa El Hentati1,2*,
Mohamed Ben Hamouda1 and Ali Chriki2
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1Regional center of agricultural research and
development on north-east, Po Box 122, 2090 Mornag, Institution of
Agricultural research and higher education, Tunisia; 2Faculty
of Sciences of Bizerta, 7021 Jarzouna, University of Carthage, Tunisia
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Abstract |
In this study, random amplified polymorphic
DNA-polymerase chain reaction (RAPD-PCR) analysis was used to assess the
genetic difference and gene flow among four sheep populations belonging
to two native Tunisian breeds (the Barbarine and the Western thin tail).
A total of 96 samples were typed using eight RAPD primers. 62 bands were
scored, of which 44 bands (70.97%) were polymorphic. The unweighed
pair-group method with arithmetic average (UPGMA) and principal
component analysis (PCA) did not show a clear differentiation between
populations within breeds. The coefficient of gene differentiation (Gst)
between populations was relatively low. Analysis of molecular variance
(AMOVA) showed that there was a genetic variation of 28.79% (FCT=
0.2879; P<0.001) among the breeds, 4.10% among populations within breeds
(FSC= 0.0576; P<0.001)) and 67.10% (FST= 0.3289;
P<0.001) within populations. The level of gene migration between
populations within breeds is quite high to homogenizing them.
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Keywords:
Genetic differentiation; RAPD-PCR; sheep; geographic
areas |
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To cite this article:
El Hentati H, M Ben
Hamouda and A Chriki,
2013.
Genetic differentiation and gene flow between four Tunisian sheep
populations (Ovis aries) using random amplified polymorphic
DNA-polymerase chain reaction (RAPD-PCR) analysis.
Res.
Opin. Anim. Vet. Sci., 3(6), 149-152. |
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