Determination of normal gastric emptying time and small intestinal transit time are useful in detecting gastrointestinal motility disorders and partial obstructions of the pylorus or small intestine. This study was conducted to evaluate gastrointestinal transit time in Persian squirrel. Twelve clinically healthy adult Persian squirrels were prepared and kept for three weeks prior to study. After eighteen hour fasting, animals were tranquilized by using Acepromazine (1mg/kg). Plain lateral and ventrodorsal radiographs were obtained. 6 ml/kg BW of a 40% w/v barium sulfate suspension was administered orally to the squirrels. Lateral and ventrodorsal radiographs were taken at zero, 10, 20 and 30 minutes after barium sulfate suspension administration and then in 30 minutes intervals until eight hours after swallowing, and then at nine, 10, 12 and 24 hours. Blood tests were performed 5 days after the study. Contrast medium was noted in the small intestine immediately after administration in two of the twelve (16.66%) squirrels. Early gastric emptying time was started at 9.16±6.68 minutes. Delay gastric emptying time was started at 332.50±37.20 minutes. Early small intestine transit time was started at 50.00±19.54 minutes. Delay small intestine transit time was started at 475.00±38.01 minutes. There was no contrast media in large intestine after twenty forth hour radiographs. It is concluded that this dosage of barium sulfate suspension gave a diagnostic opacification of upper gastrointestinal tract in Persian squirrel.