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Sperm concentration influences the post-thaw
livability of frozen goat semen
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Edeneil Jerome P. Valete1,3,
Lerma C. Ocampo1,3*, Flocerfida P. Aquino,1
Kristina J. Cruz3 and
Marlon B. Ocampo1,2 |
1Philippine
Carabao Center – Reproductive Biotechnology Unit, Science City of Muñoz,
Nueva Ecija; 2College of Veterinary Science and Medicine,
Central Luzon State University, Science City of Munoz, Nueva Ecija;
3Department of Biological Sciences, Central Luzon State
University, Science City of Muñoz, Nueva Ecija, Philippines 3120
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Abstract |
In this study, goat semen was
cryopreserved in a reduced concentration of
75×106, 50×106 and 25×106
sperm/ml and evaluated based on post-thaw motility and livability. The
ejaculated semen samples were collected using an artificial vagina,
extended with an egg yolk Tris extender, cryopreserved in straws using
glycerol (7%) and egg yolk (5%) as permeating and non-permeating
cryoprotectants in LN2. Post-thaw semen evaluation showed
motility scores of 36.6±2.13%, 35.4±2.04%, 27.0±3.0% and 19.2±2.78% for
the control, T-A (75×106), T-B (50×106) and T-C (25×106),
respectively. The mean post-thaw percentage livability obtained in the
control (60.6±4.39) was higher than T-A (42.86±1.83), T-B (39.36±2.86)
and T-C (19.2±5.27). The percentage abnormal sperm in all treatment
groups were less than 10%, indicating that the method used for freezing
of goat semen in reduced concentration could be useful in artificial
insemination and in vitro
fertilization studies. The reduced sperm motility and livability after
thawing merits further studies on the factors influencing the
survivability of sperm during cryopreservation in creating a
standardized and better freezing protocol for goat semen.
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Keywords:
Cryopreservation; livability; motility; semen
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To cite this article:
Valete EJP, Ocampo
LC, Aquino FP, Cruz KJ and Ocampo MB, 2016.
Sperm concentration influences the post-thaw
livability of frozen goat semen.
Res.
Opin. Anim. Vet. Sci., 6(5): 158-164. |
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