This study evaluated hepatotoxicity induced by potassium dichromate in chickens via drinking water. Seventy-two Hyland Brown male chickens were randomly divided into 4 groups (18 chickens each group): Control group, low-dose (2%LD₅₀) Cr(VI) group, middle-dose (6%LD₅₀) Cr(VI) group, high-dose (10%LD₅₀) Cr(VI) group. Potassium dichromate was given orally via drinking water to the chickens for 42 day. The control group was treated with the same volume of distilled water. Blood biochemical indexes (ALT, AST, GGT), antioxidant indicators (SOD, GSH and MDA) in liver homogenates and pathologic changes in tissues were detected at 14, 28 and 42 day respectively. The levels of serum biochemical indexes (ALT, AST, GGT) increased significantly (P<0.05) in Cr(VI)-treated groups. The activities of SOD and GSH decreased significantly (P<0.05) in the Cr(VI)-treated groups while the content of MDA increased significantly (P<0.05) in the Cr(VI)-treated groups compared to control with the increasing dose and time. The cells of the liver treated with Cr(VI) were severely dilated and the liver appeared severe inflamed and necrotic. The results indicated that Cr(VI) can lead to hepatotoxicity damage in chicken with time and dose-dependent relationship.