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PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
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An experimental model for study of the renal protective activity of corn
silk against dosage induced By
MDMA using in situ rat renal
system |
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1*Mohammad
Karami, 2Mohammad Shokerzadeh, 3Farshad Naghshvar
, 4Shahram
Ala, 5Reza Fezbakhsh,
6Anahita
Nosrati A and 7Marjan
Makhloogh
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1Department
of Toxicopharmacology , School of Pharmacy, Mazandaran University of
Medical Sciences, Sari, Iran; 2Department of
Toxicopharmacology , School of Pharmacy, Mazandaran University of
Medical Sciences, Sari, Iran; 3Pathology Department,
School of Medical, Mazandaran University of Medical Sciences ,
Sari, Iran; 4Department of Clinical pharmacy, School of
Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran; 5Toxicopharmacology,
School of Pharmacy, Mazandaran University of Medical Sciences, Sari,
Iran; 6Pathology Dept., School
of Medicine, Mazandaran University
of Medical Sciences, Sari ,Iran; 7Professional
Doctorate of Veterinary,
Institute of experimental animal research(IEAR), Mazandaran University
of Medical Sciences, Sari, Iran
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| Abstract |
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Corn silk (CS) is widely used in Iranian traditional medicine. The aim
of present study was to investigate renalprotective activity of CS by
Isolated Rat Renal Perfusion System (IRRP) Phenol and flavonoid contents
were measured by Folin Ciocalteu and AlCl3 assays,
respectively.
Hydro alcoholic extract of
Corn silk
(10, 20, 40 and 100 mg/kg)
was studied for its renal protective activity
by
IRRP.
Phenol and flavonoid contents of the extract were determined as gallic
acid and quercetin equivalents from a calibration curve, respectively.
IRLP system is ideal for studying biochemical alterations of chemicals
with minimum neural-hormonal effects. In this study, the renal was
perfused with Kerbs-Henseleit buffer, containing different concentration
of hydro
alcoholic extract of
Corn silk
(10, 20, 40, 50,100mg/kg) were added to the buffer and perfused for 2
hours. During the perfusion, many factors
including Urea and creatinine levels and concentration of GSH was
assessed as indicator of renal viability. Consequently, sections of
renal tissue were examined for any histo-pathological changes. The
results showed that histo-pathological changes in renal tissues were
related in a dose-dependent manner to hydro
alcoholic extract of
Corn silk concentrations. Doses of 50, 100 mg/kg caused a significant
(P<0.05) histo-pathological changes. Level of GSH of sampels of perfused
of hydro alcoholic extract of CS were increased compared with the
positive control group. We concluded that renal protective effect of CS
may be due to the decreased lipid peroxidation, although other
mechanisms may also be involved.
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Keywords:
Corn silk; Renal perfusion; Kerbs-Henseleit buffer; Histo-pathological
changes; Glutathione |
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To cite this article:
Karami M, M Shokerzadeh, F Naghshvar, S Ala, R Fezbakhsh,
A Nosrati A and
M Makhloogh,
2013.
An experimental model for study of the renal protective activity of corn
silk against dosage induced by
mdma using in situ rat renal
system.
Res.
Opin. Anim. Vet. Sci., 3(5), 143-148. |
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