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PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
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Comparison of an Alzheimer
disease drug ability to bind acetylcholinesterase using both
electrochemical and spectrophotometric assays
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Miroslav Pohanka1,2*,
Vojtech Adam3,4 and Rene Kizek3,4
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1Faculty of Military Health
Sciences, University of Defence, Trebesska 1575, CZ-500 01 Hradec
Kralove, Czech Republic, European Union; 2Karel English
College in Brno, Sujanovo namesti 356/1, 60200 Brno, Czech Republic;
3Central European Institute of Technology, Brno University of
Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic, European
Union; 4Department of Chemistry and Biochemistry, Faculty of
Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech
Republic, European Union
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Abstract |
Acetylcholinesterase (AChE) is an enzyme playing important role in
termination of neurotransmission within cholinergic system. The enzyme
is targeted by many compounds including nerve agents used for chemical
warfare, natural toxins and drugs for Alzheimer disease. Thousands of
new inhibitors are prepared for pharmaceutical purposes over the year
and necessity to characterize them is given for this reason. In this
work, standard spectrophotometric test is compared to electrochemical
test in order to assay AChE activity and measure its inhibition. The
both test were performed for characterization of an Alzheimer disease
drug galantamine. The spectrophotometric test was based on standard
Ellman´s reaction. Electrochemical test used enzymatic hydrolysis to
acetylthiocholine with consequent electrochemical oxidation. In the
experiments, we proved that electrochemical test was able to reach
better limit of detection for galantamine than the standard
spectrophotometric test. Calculated median inhibitory concentration was
same for electrochemical and spectrophotometric tests. Interference in
the assay was tested as well. We proved that electrochemical test is
reliable and more sensitive than the standard spectrophotometric test.
The electrochemical test would be preferred when median inhibitory
concentration is the assayed output.
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Keywords:
acetylcholinesterase; Alzheimer disease; myasthenia
gravis; huperzine; galantamine; donepezil; biosensor |
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To cite this article:
Pohanka
M, V Adam and R Kizek, 2014.
Comparison of an Alzheimer
disease drug ability to bind acetylcholinesterase using both
electrochemical and spectrophotometric assays.
Res.
Opin.
Anim. Vet. Sci., 4(4): 203-207. |
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