Association between different dietary protein levels and susceptibility to A. caninum infestation in puppies was investigated for a period of ten weeks. In the investigation, thirteen puppies were divided into four groups: A, B, C and D; each with three puppies except group B which had four. Puppies in groups A and B were each infected orally with 500 A. caninum larvae obtained from naturally infected dog whereas puppies in groups C and D were not infected with A. caninum. Groups A and C were maintained on balanced commercial diet while groups B and D were maintained on unbalanced locally made diet for the whole period of the study. Body weight gain, egg per gram count, packed cell volume and hemoglobin content were monitored for a period of ten weeks. The results showed that infected with unbalanced diet puppies expressed more severe clinical signs and had statistically significant higher (P<0.05) mortalities and intestinal worm burden than the infected with balanced diet puppies. Mean worm burden was 352 ± 25.86 for the balanced diet fed group and 465 ± 11.57 for unbalanced diet fed group. The infected with balanced diet fed group had statistically significant higher (P<0.05) weight gain than the infected with unbalanced diet fed group. Mean weights were 8.27 ± 0.65 kg for balanced diet fed group and 3.30 ± 0.16 kg for unbalanced diet fed group. The infected with balanced diet fed group had statistically significant higher (P<0.05) PCV values than the infected with unbalanced diet fed group. Mean PCV were 25.34 ± 1.45% for the infected with balanced diet fed group and 15.73 ± 0.64% for the infected with unbalanced diet fed group. For hemoglobin content, the infected with balanced diet fed group had statistically significant higher (P<0.05) Hb values than the infected with unbalanced diet fed group. Mean Hb was 9.89 ± 0.43mg/dl for infected with balanced diet fed group and 8.07 ± 0.19mg/dl for infected with unbalanced diet fed group. For EPG counts, the infected with balanced diet fed group had lower EPG counts than the infected with unbalanced diet fed group, however, this result was not statistically significant (P>0.05). Means EPG was 8,050 ± 949 for infected with balanced diet fed group and 13,570 ± 2811 for infected with unbalanced diet fed group. From this study, it was concluded that undernourished puppies are more susceptible to A. caninum infection and developed more severe clinical signs than puppies in good nutritional plane. In addition to regular deworming, feeding of nutritionally balanced diet should be recommended to dog keepers as ways of controlling A. caninum in dogs in poor income countries where helminthiasis remains a problem.