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Molecular detection of Theileria ovis in goat in Tehran province,
Iran
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Mahdi
Khodaveisi1, Sadegh Rahbari1*, Parviz Shayan2
and Nasser Hoghooghi Rad1 |
1Department of Parasitology, Faculty of Specialized
Veterinary Sciences, Science and Research Branch, Islamic Azad
University, Tehran, Iran, 2Department of Parasitology,
Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
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Abstract |
Theileriosis is
distributed worldwide and its prevalence leads to sever economical
losses among farm animal. This study was conducted to determine
Theileria species in goat. Blood samples from 400 randomly selected
healthy goats were examined using parasitological method and the genomic
DNA was extracted from blood and amplified using common primers
targeting to 18S rRNA of Theileria spp, subsequently semi-nested
PCR
analyses with specific primers for Theileria lestoquardi ,T. annulata,
and T. ovis. The result showed that 1.7% (7/400) cases were
positive for the presence of Theileria spp. in microscopical
examination, and 6% (24/400) positive for Theileria ovis in
semi-nested PCR. The
observation showed that there was no other DNA of protozoan parasites
existed in the blood samples of goats in this region. It can be
concluded that Theileria ovis is detectable in goats without any
clinical manifestation.
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Keywords:
Theileria ovis; Semi-nested PCR; goat; Iran |
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To cite this article:
Khodaveisi M, S Rahbari, P Shayan
and NH Rad, 2015.
Molecular detection of
Theileria ovis in goat in Tehran province, Iran.
Res.
Opin.
Anim. Vet. Sci., 5(12): 489-493. |
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