PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343


Healing effect of Adiantumcapillus veneris on surgical wound in rat
Nasim Roodbari1*, Amir Sotoudeh2, Amirali Jahanshahi3 and Mohammad Ashrafzadeh Takhtfooladi3
1Faculty of Experimental Science, Kahnooj Branch, Islamic Azad University, Kerman, Iran
2Faculty of Veterinary Science, Kahnooj Branch, Islamic Azad University, Kerman, Iran
3Department of Surgery, Science and Research Branch, Islamic Azad University, Tehran, Iran
 
Abstract

The aim of this study was to investigate the potential effect of Adiantumcapillus veneris extract on the healing of surgical wound. Forty eight male Wister rats were divided into 3 groups of 16 rats each. Group I received ointment containing the Adiantumcapillus veneris extract, group II received simple ointment twice a day until the animals were euthanized. The control group did not receive any medication. Animals were kept under observation for a period of 21 days. Histological evaluation of wound healing was done on 3, 5, 14 and 21 day after surgery. For this purpose four animals in each group were euthanized at the set time. The tissues were stained with haematoxylin and eosin and the number of inflammatory cells, new fibroblast cells, new capillary formation, epithelial cells and collagen fibres formation were counted. Histological analyses revealed that in the group І and ІІ a few of the inflammatory cells and fibroblast-like cells on day 3 were observed. On day 5, a continuous incomplete epidermal layer was observed under the crust, and the number of inflammatory cells decreased slightly, there was a distinct increase in fibroblast-like cells, collagen formation and new vessels formation from day 3. On day 14, a complete epidermal layer with multiple layers of spinous cells was observed, and the number of inflammatory cells decreased remarkably. On day 21, group І and ІІ showed relatively well organized epidermal layer, increased number of matured fibroblasts, remarkable degree of neo vascularisation and epithelization. The score for inflammation was the highest on day 3, and thereafter, it decreased gradually up to day 14. The score for the proliferation of the fibroblast like cells increased from days 3 to 14, and a sharp increase in the score was observed from day 5 to 14. The score for collagen formation was same as proliferation of the fibroblast like cells. The score for the epidermal cell regeneration cells increased from days 5 to 14, and a sharp increase in the score was observed from day 14 to 21. The changes observed in these 5 parameters were significant. The Adiantumcapillus veneris extract showed remarkable wound healing and it may be suggested for treating various types of wounds in human beings.

Keywords: Adiantumcapillus veneris; wounds healing; histology; rat
 
To cite this article: Roodbari N, A Sotoudeh, A Jahanshahi and MA Takhtfooladi, 2012. Healing effect of Adiantumcapillus veneris on surgical wound in rat. Res. Opin. Anim. Vet. Sci., 2(12), 591-595.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

Home  |  Archive  |  Instructions  |  Submission  |  Editorial Board  |  Sample Paper

All rights reserved © roavs.com