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PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
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Healing effect of
Adiantumcapillus veneris on surgical wound in rat |
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Nasim Roodbari1*, Amir Sotoudeh2, Amirali
Jahanshahi3 and Mohammad Ashrafzadeh Takhtfooladi3 |
1Faculty
of Experimental Science, Kahnooj Branch, Islamic
Azad
University, Kerman, Iran
2Faculty of Veterinary Science, Kahnooj Branch, Islamic
Azad
University, Kerman, Iran
3Department of Surgery, Science and Research Branch,
Islamic Azad
University, Tehran, Iran |
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Abstract |
The aim of this study was to investigate the potential effect of
Adiantumcapillus veneris
extract on the healing of surgical wound. Forty eight male Wister rats
were divided into 3 groups of 16 rats each. Group I received ointment
containing the Adiantumcapillus
veneris extract, group II received simple ointment twice a day until
the animals were euthanized. The control group did not receive any
medication. Animals were kept under observation for a period of 21 days.
Histological evaluation of wound healing was done on 3, 5, 14 and 21 day
after surgery. For this purpose four animals in each group were
euthanized at the set time. The tissues were stained with haematoxylin
and eosin and the number of inflammatory cells, new fibroblast cells,
new capillary formation, epithelial cells and collagen fibres formation
were counted. Histological analyses revealed that in the group І and ІІ
a few of the inflammatory cells and fibroblast-like cells on day 3 were
observed. On day 5, a continuous incomplete epidermal layer was observed
under the crust, and the number of inflammatory cells decreased
slightly, there was a distinct increase in fibroblast-like cells,
collagen formation and new vessels formation from day 3. On day 14, a
complete epidermal layer with multiple layers of spinous cells was
observed, and the number of inflammatory cells decreased remarkably. On
day 21, group І and ІІ showed relatively well organized epidermal layer,
increased number of matured fibroblasts, remarkable degree of neo
vascularisation and epithelization. The score for inflammation was the
highest on day 3, and thereafter, it decreased gradually up to day 14.
The score for the proliferation of the fibroblast like cells increased
from days 3 to 14, and a sharp increase in the score was observed from
day 5 to 14. The score for collagen formation was same as proliferation
of the fibroblast like cells. The score for the epidermal cell
regeneration cells increased from days 5 to 14, and a sharp increase in
the score was observed from day 14 to 21. The changes observed in these
5 parameters were significant. The
Adiantumcapillus veneris
extract showed remarkable wound healing and it may be suggested for
treating various types of wounds in human beings.
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Keywords:
Adiantumcapillus veneris;
wounds healing; histology; rat
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To cite this article:
Roodbari N, A Sotoudeh, A Jahanshahi and MA Takhtfooladi,
2012.
Healing effect of Adiantumcapillus veneris on surgical wound in rat.
Res.
Opin. Anim. Vet. Sci., 2(12), 591-595. |
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