The present study was conducted to investigate the effects of CreAMINO^® (96% Guanidinoacetic acid) addition into the broiler on growth performance, carcass trait, chemical composition of meat and molecular investigation of the expression of Myogenin, Myostatin and IGF-1 genes. Three hundred fifty one-day-old Ross 308 chicks were allocated to seven dietary treatments with 5 replicates containing 10 birds in each. Each feeding phase (starter, grower or finisher) was extended for two weeks. The treatments were control diet (without CreAMINO®), the 2nd, 3rd , 4th and 5th treatments were the same as control in addition to CreAMINO® was added on top with 0.6 g/kg to the control diet in starter only, grower only, finisher only and whole rearing period for (2nd, 3rd , 4th and 5th treatment, respectively). A control reduced metabolizable energy and crude protein diet (50 kcal/kg and 0.5%, respectively) was formulated to be the 6th treatment and CreAMINO® was added to the control reduced diet on top with 0.6 g/kg to be the 7th treatment. The results indicated that supplementation of broiler diets with CreAMINO^® significantly improved bird performance. The best performance was seen when CreAMINO^® was fed during the whole rearing period compared to control, rearing stages and reduced feed contents. Addition of CreAMINO^® to the control. Adding CreAMINO^® into reduced feed contents, fully compensated bird performance. CreAMINO^® supplementation significantly improved carcass dressing, breast meat yield, reduced abdominal fat, and increased the protein content of the breast muscle. Moreover, feeding CreAMINO^® had no negative effect on kidney function tests. CreAMINO® showed a powerful anabolic effect on skeletal muscle, indicated by the up regulation of Myogenin and IGF-1 genes and down regulation of Myostatin gene expression in the pectoral muscle. In conclusion, CreAMINO® improved broiler growth performance, carcass traits and had sparing effect on energy and protein in broiler diet.