The objective of this experiment was to determine the effects of dietary supplementation of golden flaxseed powder on some blood quality biochemical in male and female broiler. A total of 180 unsexed, Ross broiler chicks were randomly divided into four treatment groups. Chicks in each treatment group were sub-divided into three replicates (15 chicks for each replicate). The treatment groups were as follows: T1: Control group; T2: fed on a diet supplemented with 12% flaxseed; T3: fed on a diet supplemented with 14% flaxseed; T4: fed on a diet supplemented with 16% flaxseed. The result showed that serum protein and albumin decreased significantly (P<0.05) in male and female broiler fed with 16% flax seeds. Further AST and ALP increased significantly (P<0.01) in male and female broiler fed with the highest level of flaxseed (16%). All other parameters remained unchanged (P>0.05) between control and experimental groups. It can be concluded from the study that the use of flaxseed powder at higher concentrations in the diet led to a reduction of blood protein and increased liver enzymes in male and female broiler.