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PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
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Responses of inflammatory cytokines in non-pregnant Boer does inoculated
with Corynebacterium pseudotuberculosis via various routes
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Othman,
A.M1, Jesse, F.F.A1,2*, Adza- Rina, M.N2,
Ilyasu, Y1, Zamri-Saad,
M2, Wahid, A.H2,
Saharee, A.A2 and Mohd-Azmi, M.L |
1Department of Veterinary Clinical studies; 2Research
Centre for Ruminant Diseases Faculty of Veterinary Medicine, Universiti
Putra Malaysia, Serdang, Malaysia
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Abstract |
Corynebacterium pseudotuberculosis is the
causative agent of caseous lymphadenitis in sheep and goats and is
characterized by the development of pyogranulomas in the lymph nodes and
organs. This study was designed to measure the concentration of
inflammatory cytokines (IL-1β & IL-6) in experimental non-pregnant Boer
does inoculated with Corynebacterium pseudotuberculosis through
various routes. Little is known about the concentration of IL-1β and
IL-6 in different routes of infection caused by the organism. A total of
twenty healthy non-pregnant Boer does were divided into 4 groups (A-D)
of 5 does per groups. Three groups (A-C) were inoculated with 107cfu/1ml
of live Corynebacterium pseudotuberculosis through intradermal,
intranasal and oral routes respectively, while group D was kept
unexposed. Following infection, blood samples were collected from the
jugular vein between three days interval period for the analysis of
IL-1β and IL-6. A significant increase (P<0.05) in IL-1β was observed
through intranasal, intradermal and oral route compared to the control
group. Significant increase in IL-1β and IL-6 was observed through
intranasal route compared to other routes. This study, therefore,
highlights the effects of inflammatory cytokines (IL-1β andIL-6) in
caseous lymphadenitis infection.
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Keywords:
Caseous lymiphadeinitis;
C. pseudotuberculosis; non-gravid Boer does; interleukin-1β;
Interleukin-6 |
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To cite this article:
Othman AM, FFA Jesse, MN Adza-Rina, Y Ilyasu, M Zamri-Saad, AH Wahid, AA
Saharee, ML Mohd-Azmi, 2014.
Responses of inflammatory cytokines in non- pregnant
boer does inoculated with Corynebacterium pseudotu berculosis via
various routes.
Res.
Opin. Anim. Vet. Sci., 4(11): 597-600. |
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