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Use of platelet activating factor for inducing bull sperm capacitation
in vitro: A novel approach |
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Subodh Kumar1*, Yathish, Hirenallur Maheshwarrappa1,
Balasubramaniam Sivamani1, Subuhi Khan1, Nihar
Ranjan Sahoo1, Anuj Chauhan1, Deepak Sharma1
and Arjava Sharma2
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1Division
of Animal Genetics, Indian Veterinary Research Institute, Izatnagar
(U.P.) India
2Project
Directorate on Cattle, Post Box # 17, Grass Farm Road, Meerut Cantt.
(U.P.) India
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Abstract |
Several chemicals viz., hyamine, Ca+2 ionophore, high ionic
strength (HIS) media, glycosaminoglycans (GAGs), lysophosphatidyl
choline (LPC), lipids, chondroitin sulphate, heparin sulphate, caffiene,
oviductal fluid, follicular fluid etc. have been reported to induce
in vitro capacitation of
spermatozoa. Off late, a new potent vasoactive phospholipid named
Platelet Activating Factor (PAF) has been reported to have multifold
involvement in a wide spectrum of reproductive activities in both the
sexes. Though the exact role of PAF on sperm function is not very clear
yet it has been observed that it plays a significant role in sperm
motility, acrosome reaction and the fertilization process. A special
attribute of this source of capacitation is that it requires very little
time for acrosome reaction and the motility of spermatozoa is maintained
at a reasonable level. The
results of work done in our laboratory indicated that PAF treatment of
spermatozoa caused a significant increase in acrosome reaction, which is
an indirect yardstick of capacitation and the motility decrement was
much within the permissible limits. Despite increment, all the sperm
abnormalities were pretty much within the permissible limits. The stress
indicator enzymes viz. seminal GOT and GPT were not affected with PAF
treatment of spermatozoa and the proteolytic enzyme- hyaluronidase had a
significant increase. Based on these precepts, PAF can be recommended as
an ideal substance to induce capacitation and improve fertilizing
ability of spermatozoa for in vitro fertilization (IVF).
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Keywords:
Buffalo;
cattle; in vitro capacitation; platelet activating factor; sperm |
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To cite this article:
Kumar S, Yathish HM, Sivamani B, Khan S, Sahoo NR, Chauhan A, Sharma D
and Sharma A, 2013. Use of platelet activating factor for inducing bull
sperm capacitation in vitro: A novel approach. Res. Opin. Anim. Vet.
Sci., 3(10), 348-355. |
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