E-ISSN 2223-0343

Phytochemical composition and insecticidal effect of essential oil from Origanum vulgare L.

Tunay Karan1* and Seyda Simsek2

1Plant Research Laboratory, Department of Molecular Biology and Genetic, Faculty of Arts and Sciences, Gaziosmanpasa University, 60240 Tokat, Turkey; 2Department of Plant Protection, Faculty of Agriculture, Bozok University, Yozgat, Turkey


Origanum genus belonging to Lamiaceae family has been used extensively throughout the world. Air-dried aerial part of Origanum vulgare L. was subjected to steam distillation to yield the essential oil of which the compounds were elucidated by GC-MS analysis. The main constituents of essential oil were determined as thymol (40.35%), p-cymene (17.32%), γ-terpinene (15.66%), and carvacrol (12.15%). The insecticidal activity of essential oil was executed against Sitophilus oryzae (L.) (Coleoptera: Curculionidae) and Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae) by fumigation method. It was presented that the essential oil was highly effective against both species. After treatment of essential oil on S. oryzae, 72% and 88% mortality were observed at 48 hours (h) and 72 h respectively. The mortality of R. dominica was determined as 94% and 96% at 48 h and 72 h respectively. Median lethal concentration (LC50) values were found for S. oryzae as 0.086 µL /mL and 0.057 µL /mL at 24 h and 48 h respectively. Moreover, these values (LC50) were presented as 0.11 µL /mL and 0.065 µL /mL at 24 h and 48 h respectively for R. dominica. Hence, the essential oil of O. vulgare could be useful for the managing field populations of S. oryzae and R. dominica.

Keywords: Origanum vulgare; essential oil; insecticidal activity
To cite this article: Karan T and S Simsek, 2018. Phytochemical composition and insecticidal effect of essential oil from Origanum vulgare L.. Res. Opin. Anim. Vet. Sci., 8(1): 19-23.

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