The study optimized and established an efficient protocol for in vitro production of bovine pre-implantation embryos. Specifically, it developed a protocol that will yield higher blastocyst rate and better embryo quality using the modified Synthetic Oviductal Fluid and modified Potassium Simplex Optimization Medium culture media. Aspirated and selected oocytes from slaughterhouse-derived ovaries were placed into an in vitro maturation medium for 22 h.  In vitro fertilization was allowed for 16 to 18 h and presumptive zygotes were washed and placed on the six different treatment culture media containing different combinations of modified Synthetic Oviductal Fluid and modified Potassium Simplex Optimization Medium culture media and supplemented with bovine serum albumin and fetal calf serum. In sequential culture media, embryos were transferred after 3-4 days to the second treatment culture media. Cleavage rate and development to blastocyst was determined at 2 and 7 days after in vitro culture, respectively.  Blastocysts were stained with Hoechst 33342-Propium Iodide differential staining to assess embryo quality. The rate of cleavage was not significantly different among all treatments; however, sequential culture of embryos in modified Synthetic Oviductal Fluid + 3mg/ml BSA for 1-3 days and Potassium Simplex Optimization Medium + 5% FCS for 4-7 days yielded higher blastocyst development and blastocyst cell number. Based on the results, it can be concluded that the use of modified Potassium Simplex Optimization Medium supplemented with fetal calf serum in the later part of in vitro culture is beneficial in bovine embryo development.