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Factors influencing the maturation, fertilization
and development of swamp buffalo oocyte
in vitro
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Lerma C. Ocampo1,2, Lucia M. Rigos2
and Marlon B. Ocampo |
1Reproductive Biotechnology Unit, Philippine Carabao
Center, Science City of Muñoz, Nueva Ecija, Philippines 3120, 2College
of Veterinary Science and Medicine, Central Luzon State University,
Science City of Muñoz, Nueva Ecija, Philippines 3120
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Abstract |
Buffalo ovaries (n=610)
were collected from the abattoir and the oocytes recovered were matured,
fertilized and the resulting zygotes were cultured in vitro. In
Experiment 1, the maturation and fertilization rates of O1-O2
oocytes were higher than O3-O5 oocytes. In
Experiment 2, the two batches of TCM-199 medium used with different
hormonal supplementation did not influence the maturation and
fertilization of the oocytes at 70.7-86.7% and 59.4-66.0% respectively.
In Experiment 3, bull variability was observed in terms of their
capacity for fertilization. In Experiment 4, higher fertilization rate
was observed in response to caffeine (3–10 mg/ml) compared to control.
In Experiment 5, 1 × 106 sperm/ml concentration during
fertilization had a lower penetration rate (43.0%) than 5, 10 × 106
sperm/ml concentration (68.4- 71.0%). In Experiment 6, development to
the blastocyst stage in TCM-199 or mSOF medium with or without cumulus
cell monolayer had no significant difference (11.5-22.2%) with total
cell count ranging from 84.6±11.2 to
91.4±12.2. Overall, the results showed the importance of cumulus
cells in the acquisition of developmental competence of oocytes maturing
in vitro and its subsequent fertilization. That, selection of
appropriate bull, its optimum sperm concentration, the choice of
capacitating agent and its concentration, the type of medium for culture
of resulting zygotes is critical for successful application of in
vitro maturation, fertilization and culturing for mass production of
buffalo embryos.
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Keywords:
Embryos; fertilization;
maturation; oocytes
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To cite this article:
Ocampo LC, LM Rigos and
MB Ocampo, 2016.
Factors
influencing the maturation, fertilization and development of swamp
buffalo oocyte in vitro.
Res.
Opin.
Anim. Vet. Sci., 6(1): 24-31. |
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